Chapter 354 Brother Donkey: Am I the first driving force for technological development?
University of Science and Technology of China.
Life Medicine Building.
Medicinal chemistry laboratory on the fourth floor.
Click——
With the sound of opening the door, Xu Yun hurriedly walked in from outside the house wearing a lab coat:
"Old Qiu, what's going on?"
As a result, before he finished speaking, he noticed the figure of another person in the room:
"Huh? Academician Zhao?"
That's right.
At this time, in addition to the tool man Qiu Sheng, there was also another old acquaintance of Xu Yun sitting in the laboratory:
Academician Zhao Zhengguo!
He is now the director of the Experimental Physics Center of the Institute of High Energy Physics, Chinese Academy of Sciences, and the person in charge of the original experiment on Λ hyperons.
He and Pan Shuai relied on the particle orbit calculated by Xu Yun to capture a brand new Λ hyperon.
"Well, Xiao Xu, good afternoon."
Zhao Zhengguo greeted Xu Yun, pointed at Qiu Sheng with a smile, and explained:
"I want to talk to you about something today. When I first came to see you, Xiao Qiu said you would be there soon, so I will stay here."
"But my matter is not urgent. You two can deal with your matters first. Do you mind if I stay here and listen?"
Xu Yun admired Zhao Zhengguo very much, so he waved his hand quickly:
"I don't mind, of course I don't mind - besides, how many people in China dare to kick you out?"
After giving Zhao Zhengguo a small compliment, Xu Yun turned his attention to Qiu Sheng:
"Lao Qiu, what's going on with you?"
Qiu Sheng glanced at Zhao Zhengguo, who was sitting and listening quietly, and waved to Xu Yun:
"Come and see."
Xu Yun hummed and walked over quickly.
At this time, there was a stack of thick paper reports on the table in front of Qiu Sheng. When Xu Yun approached, Qiu Sheng handed a few pages to him:
"Here, this."
Xu Yun took it and looked it up on the spot:
"Easy bacteria strain decomposition rate and CCK-8 test report?"
Students who were bacteria in their previous lives should all know this.
There are many methods for testing bacterial activity in the laboratory, but only a few are commonly used.
For example, the chemiluminescence method is the ATP luminescence method.
Adenosine triphosphate is composed of adenine, ribose and three phosphate groups. It releases more energy when hydrolyzed and is the most direct source of energy in the body.
Exogenous firefly luciferin or luciferase will oxidize with ATP in the cell to generate photons.
Therefore, ATP content can be detected by monitoring luminosity to analyze cell activity and proliferation.
In addition to the ATP luminescence method.
CCK-8 is also a common and efficient detection method.
Its main component is water-soluble tetrazolium salt SST-8(2-(2-methoxy-4-niphenyl)-3-(4-niphenyl)-5-(2,4-disulfobenzene) )-2H-tetrazole monosodium salt), is a compound similar to MTT.
In the presence of electronic coupling reagents.
It can be reduced by some dehydrogenases in mitochondria to produce orange-yellow water-soluble formazan.
The formazan generated after SST-8 is bioreduced by intracellular dehydrogenase can be directly dissolved in the culture medium. The more and faster the cells proliferate, the darker the color will be.
"Phosphatidylserine is mainly distributed in the inner layer of the plasma membrane without asymmetric changes."
"The reduction potential value shifted by +228mV"
"The spectrophotometer readings are 0.434, 0.437, 0.432 respectively"
Xu Yun read on line by line, each item of figures made him a little happy.
When he first discovered Yi'an bacteria, Tian Liangwei generously sent Xu Yun a Micro-Matrix and a 350RPM orbital shaker.
Although Tian Liangwei's purpose is not "pure", part of the reason is to let Xu Yun and others be the guinea pigs for microfluidic interface nanoliter injection technology and high polymer feeding method.
However, the accuracy improvements brought by these two devices to the laboratory are also visible to the naked eye.
After a few minutes.
Xu Yun slowly raised his head, rubbed the report paper gently with his thumb, and asked Qiu Sheng:
"Lao Qiu, according to the test results, the activity of Yi'an bacteria is much higher than other strains of Arthrospora?"
Qiu Sheng nodded heavily, picked up a can of yogurt from the table and took a sip:
"Yes, according to experiments, the reproduction efficiency of Yi'an bacteria is extremely high."
"At a certain density, the only thing that limits their reproduction is the nurturing environment of the products of the pentose phosphate pathway, or in a sense, the number of native donkey hair."
"Also."
"Lao Xu, the activity of Yi'anbacterium is not just as simple as being higher than that of other Arthrospora species."
Xu Yun glanced at him with a frown, curled up the experimental report and knocked it on Qiu Sheng's head:
"This isn't the end of the chapter. Why are you pretending to be the Riddler? You're talking like a human being!"
"I'm just following your example. Hey, stop fighting, stop fighting!"
Qiu Sheng rubbed the back of his head and pressed his chin towards the report in Xu Yun's hand:
"Old Xu, do you still remember how Yi'an bacteria kill Neisseria and anaerobic streptococci?"
Xu Yun nodded and answered simply:
"Of course I remember, bald attack."
Neisseria and Anaerobic Streptococcus are both bacteria that are abundant in human saliva and are two of the important 'members' that cause bad breath.
Yi'anbacterium has an extremely efficient killing ability against these two types of bacteria. Its main method is to split the hyphae and use the hyphae to 'bind' the target bacteria.
This attack method is like a person constantly taking off his hair to kill the enemy, so it is also jokingly called a hair loss attack by Xu Yun and Qiu Sheng.
In the laboratory.
Seeing that Xu Yun still didn't understand what he meant, Xu Yun had to remind him again:
"Old Xu, haven't you thought of it yet?"
"The moment Yi'an bacteria sheds mycelium, to put it bluntly, it is an energy transformation, that is to say, it will spread out"
"Electrons! And lots of electrons!"
electronic.
When these two light words were passed from Qiu Sheng's mouth to Xu Yun's mind, they suddenly turned into a thunder!
In an instant.
A numb feeling suddenly rose from Xu Yun's tailbone and went straight into his mind!
More than ten seconds passed.
Xu Yuncai slowly came back to his senses, looked at Qiu Sheng blankly, and asked:
"You mean"
"Easy bacteria can be used as power generating bacteria for microbial batteries?!"
Qiu Sheng chuckled, obviously very satisfied with Xu Yun's surprise. He did not remember that when he saw the experimental results, he was even more shocked than Xu Yun at this time:
"Congratulations, you answered correctly, ten points for Azkaban!"
Xu Yun directly ignored the work of this tool man. At this moment, his mind was completely focused on Yi Anji.
There is an old saying in China that a word can wake up a dreamer.
Now Xu Yun was completely shocked by Qiu Sheng's words. He quickly linked the cause and effect together, and formed the general outline of the incident in his mind:
a month ago.
After returning to reality.
Xu Yun once organized a brainstorming session and mentioned microbial batteries and hemostatic gelatin to Qiu Sheng, Zhou Shan and others.
Especially microbial batteries, this project was directly set by Xu Yun as the next stage goal.
After all, this is a technology with the thinnest barriers among all rewards. It is so thin that if you directly propose an idea, it will not surprise others at all.
Because the concept of microbial batteries has been around for who knows how many years, the entire technology is actually very clear at the theoretical level, and the most difficult ones are only two barriers:
Powered strains and how current is collected.
But now according to what Qiu Sheng said.
Yi'an Bacteria seems to perfectly meet this requirement?
Think carefully
Seems quite reasonable?
The area where Yi'an bacteria was first discovered was the sewer with the largest traffic flow at the University of Science and Technology. It was surrounded by Nanako's lair, and there were millions of cockroaches.
Yi'an bacteria can clean a piece of pure land in that kind of area, which shows that its biological attributes must have two characteristics:
First.
Able to survive in harsh environments.
This harsh condition can be a sewer pipe, or it can also be
Storage tank for microbial batteries.
second.
It must be very efficient at cleaning.
Otherwise, before one round of cleaning is completed, the next round of sewage will come again, and it will be impossible to form the "pure land" that Xu Yun saw.
To some extent.
This is also a kind of natural selection of life evolution.
On this basis.
When Yi'an bacteria begin to devour other bacteria, the whole process requires consumption, or conversion of a lot of energy.
The way the hair structure engulfs it will generate an action potential.
Judging from Qiu Sheng's test results.
The electrical activity of Yi'an bacteria is very powerful and efficient, even far exceeding that of Shewanella bacteria, the mainstream strain in current microbial battery experiments.
Of course.
Just because Yi'anbacteria is suitable does not mean that Yi'anbacteria's microbial batteries can be produced immediately.
There are still many links before Xu Yun that need to be considered or broken through.
For example, how to provide energy to Yi'an bacteria?
You can't just let the user spit directly into the storage slot, right?
Not to mention how much electricity a mouthful of spit can generate, this behavior alone is quite disgusting
Perhaps some outdoor enthusiasts can accept this, but Xu Yun obviously cannot only take care of this group - if small microbial batteries can be released, the audience of business travelers and students will be much larger than the circle of outdoor enthusiasts.
In addition, how to collect electrons is also a technical barrier. It can only be said that it is simpler than the most difficult strain selection.
Just like this.
The collection of donkey hair is about to increase again.
Then Xu Yun put the report away and asked Qiu Sheng again:
"I understand, Lao Qiu, is there anything else you can do?"
Xu Yun just asked casually, but unexpectedly Qiu Sheng actually nodded:
"Well, there is one more thing, which is also related to the biotechnology you mentioned before - do you remember the hemostatic gelatin you mentioned?"
Xu Yun was slightly startled:
"Of course I remember. Why, you have also made progress in hemostatic gelatin?"
"Strictly speaking, it's not progress."
Qiu Sheng shook his head and explained:
"It was only during the research process that I discovered a material direction that seemed feasible."
Hearing this, Xu Yun frowned even more:
"Research process? I remember that we haven't approved a project on coagulation gelatin yet, right?"
Naturally, Xu Yun's words were not questioning Qiu Sheng's small tricks in private. His lifelong relationship with Qiu Sheng gave him some extraordinary trust in Qiu Sheng.
So what he said was indeed literal:
Hemostatic gelatin was just an idea that Xu Yun casually mentioned during brainstorming. He didn’t even conduct preliminary demonstrations, let alone start research.
Therefore, there must be no relevant equipment and materials in the laboratory at present. How could Qiu Sheng produce results out of thin air?
Seeing Xu Yun's confused look, Qiu Sheng opened the drawer and took out a test tube.
I saw that inside the test tube, there was something there
Another tuft of donkey hair.
Then Qiu Sheng shook the test tube a few times and explained:
"Lao Xu, haven't I been amplifying Yi'an bacteria during this time? I have used a lot of donkey hair as culture medium."
"Since we need to select the most suitable culture medium, we naturally need to add some other substances as control groups to optimize the most suitable amplification environment, right?"
Xu Yun nodded. Qiu Sheng's words were not difficult to understand:
"That's right.
Qiu Sheng then continued:
"As a result, one time when I was doing quantitative work, I suddenly discovered that a tuft of donkey hair was stained with some mucus. After the mucus rested with benzoquinoline for a period of time, it would form a very high-viscosity colloidal object."
"So I thought of the coagulated gelatin you mentioned, and tried to measure the longitudinal adhesion force of that substance with a rheometer, and the result was..."
"Its viscosity curve exceeds 860! It is more than six times that of 502 glue!" (End of this chapter)