Chapter 1018 Misunderstanding, wife!
Yang Ping is very relieved that Lu Xiaolu is the director of the tumor laboratory. Lu Xiaolu’s research field itself is the basic research of tumors, and this person is indeed a rare talent. The achievements he achieved before were achieved under a state of depression. Now he is completely mentally and physically. In a liberated state, it is easier to achieve scientific research results.
After returning home from get off work, Yang Ping played with his son for a while. After changing his diaper, he held him in his arms and told him the operations and procedures he had done in the hospital. The boy actually didn't cry or fuss. He was smiling widely, and his limbs were kicking around in excitement.
Xiao Su is exercising in the gym at home. Her postpartum recovery is very good. Yang Ping's mother and Mrs. Su are working together in the kitchen to cook. Professor Su and Yang Ping's father are chatting in the living room, as if they are commenting on a war movie. The family is getting along. Very harmonious.
The advantage of having a system is that you can start work at any time. After Yang Ping told his son a story, he put him on the crib. The little guy blinked his eyes. It may have been hard. He blinked a few times and fell asleep. At this time, Xiao Su finished exercising. .
"Let me watch him. You take a rest." Xiao Su said.
''I'll just squint here for a while. "Yang Ping said.
Xiao Su knew Yang Ping's habits. He liked to take advantage of fragmented time to rest, so he was as energetic as a cow all day long.
Yang Ping just squinted on the chair in the bedroom for a while, and then did what he said. He used his spare time to enter the system space laboratory, and went to the system mall to purchase various equipment according to the needs of the experiment. Soon all the hardware required for the experiment was built.
To use M13 phage to carry K factor, we must first study the structure and function of M13 phage. Only by thoroughly studying it can we better transform it and make it work for ourselves.
Through the special camera system of the electron microscope, the M13 phage appeared on the electronic screen, showing a unique and fascinating shape.
From a morphological point of view, the M13 phage appears slender and uniform, like a delicate line. The ratio of its length to diameter makes it extremely eye-catching under the microscope. The coat consists of the major coat protein p8, which are tightly packed together to form the phage's protective coating. Under a microscope, you can clearly see these layers of protein structures, which are arranged in an orderly manner and protect the internal genetic material of the phage.
For the first time, Yang Ping felt that the microbial structure assembled by this protein was so beautiful. Yang Ping couldn't help but marvel at the precision and beauty of living things. It even looked more harmonious and beautiful than any man-made machine.
The activity of M13 phage is also very distinctive. When they encounter a suitable host cell, such as E. coli containing F plasmid, they will demonstrate their ability to infect. Under a microscope, M13 phage can be observed attaching to the F pilus of E. coli via its minor coat protein p3, which is the first step in infection. Once attached, the M13 phage begins injecting its DNA into the host cell.
Once the DNA is injected, the M13 phage uses the host cell's resources to replicate. Under a microscope, it can be observed that the interior of the infected cell begins to change, and new phage particles are gradually assembled within the cell. This process is crucial to the life cycle of phages and is key to their reproduction in nature.
It is worth noting that after infecting host cells, M13 phage does not cause host cell lysis like some phages. Instead, they secrete new phage particles from infected cells, while the host cells are still able to continue growing and dividing. This property makes M13 phage uniquely valuable in biological research. But for Yang Ping, this is not an advantage, but a disadvantage.
The electronic screen continues to live broadcast the observation of the morphology and activity of M13 phage under the microscope, which is like a fascinating journey of exploration. Their delicate structure, unique infection patterns, and distinctive life cycles demonstrate the wonder of nature and the charm of biodiversity.
Such a simple virus is so sophisticated, one can only imagine how sophisticated the complex human body is.
Looking at the phage on the screen, Yang Ping fell into thinking. He couldn't wait to complete the entire experiment quickly, but he didn't expect that a whole new world would open up when he started over. If it is modified into a killer that can infect tumor cells, they will no longer infect E. coli, but will accurately adsorb to the outer membrane of tumor cells, and then inject not only DNA through their long filamentous tails, but DNA and K factor, K factor begins to work inside the cell, initiating the apoptosis of tumor cells, while DNA allows the phage to divide and grow on its own.
If M13 phages can be modified according to their goals, they will exhibit unique characteristics. They are no longer simple parasites, but become "intelligent warriors" that can actively attack tumors. This process involves sophisticated genetic engineering. Allowing these tiny creatures to play a vital role in the battle against cancer.
However, the transformation process is destined to be difficult and complicated. The process of transforming M13 phage is complex and delicate.
First, it is necessary to determine which genes or proteins need to be modified or added to achieve anti-tumor effects, which usually involves precise editing of the phage genome, including inserting, deleting or replacing specific gene segments.
But for Yang Ping, it obviously does not need to be so complicated, because he has already mastered the K factor that initiates tumor cell apoptosis. He only needs to combine the K factor with the adhesion protein on the surface of the M13 phage, and then allow the phage to recognize and contact the tumor cells. The rest of the work is a matter of course. If it is done more advanced, the K factor is mixed with the DNA inside the phage. After the phage infects the tumor cells, the K factor mixed with the DNA is injected into the tumor cells together.
The K factor can initiate the apoptosis of tumor cells and eventually kill the tumor cells. In this process, DNA can also waste materials inside the cells to divide and strengthen the phage itself.
After designing the experiment, Yang Ping handed over the rest of the work to the laboratory's robots, asking them to continuously modify the phage, and then conduct a pairing test with the phage and K factor. It is estimated that the M13 phage can obtain hundreds of millions of different phages through modification. Which of the phages can adhere to K factor and which one can identify tumor cells is a huge trial and error project. Trial and error is the specialty of the system space laboratory. Yang Ping left it to automated equipment to complete.
After coming out of the system space, Yang Ping yawned. Dinner was probably not ready yet. Xiao Su asked curiously: "Why do you feel like you have been injected with chicken blood after just sleeping for a while?"
"I'm thinking about a problem." Yang Ping said.
"What's the problem?" Xiao Su asked curiously.
"I was thinking about how phages inject DNA into cells. The phage's long tail is like a thick tube. When it finds the cell it likes, it immediately locks on it, and then inserts the tail into the cell to forcefully inject the DNA. Injected into cells, I have been thinking about this issue, so the more I think about it, the more excited I am." Yang Ping has indeed been thinking about this issue.
Xiao Su listened and wondered why this description was getting more and more wrong. Then, if she realized something, she hit Yang Ping's chest with her pink fist.
"You are so energetic! After a whole day of surgery, are you still thinking about injecting DNA?"
"Honey, I was really just thinking about injecting DNA into your body. Oh, no, I meant how bacteriophages inject DNA into cells." Yang Ping got excited and confused his words.
Misunderstanding, wife!
My thoughts are pure, and I was really thinking about scientific research just now.